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  • Cloning and identification of an angiostatic molecule IP-10/crg-2

    • 摘要：

      AIM: To obtain human and murine cDNAs encoding IFN-γ inducible protein 10 (IP-10) and cytokine responsive gene-2 (Crg-2). METHODS: The encoding genes of IP-10 and Crg-2 were amplified by RT-PCR from cultured human fibroblast cells and Balb/ c mouse liver treated by IFN-γ and TNF-α, respectively, and cloned into plasmids of pUC19 and pGEMSZf (+). RESULTS: The nucleotide sequences of the amplified DNA were confirmed by endonucleases digestion and sequencing. CONCLUSION: Recombinant IP-10/ crg-2 gene clones with 306 bp and 314 bp inserts were established for further research on biological activities and ligands of hIP-10/mCrg-2. Copyright

    • 作者：

      Zhi Guo Liu;Jing Hua Yang;Hua Zhang An;Hai Yan Wang;Feng Tian He;Zhe Yi Han;Ying Han;Han Ping Wu;Bing Xiao;代明 樊

    • 刊名：

      World Journal of Gastroenterology

    • 在线出版时间：

      1999-6