本发明提供了一种从微生物发酵液或酶转化液中同时提取α-酮戊二酸和丙酮酸的方法，涉及生物分离提取技术领域。该方法包括以下步骤：将含有α-kg和pa的微生物发酵液或酶转化液离心，去除细胞和其它可见固体；超滤去除大分子杂质；减压蒸发浓缩；酸化后采用水不溶性萃取法提取；蒸发结晶法分离α-kg粗晶和pa粗液（如果pa浓度远高于α-kg，则需蒸馏部分纯丙酮酸后进行结晶分离）；α-kg粗晶用乙酸乙酯或乙酸丁酯等不溶于水的有机溶剂洗涤，干燥粉碎，得合格的α-kg；采用高真空蒸馏（或分子蒸馏）蒸馏，得合格的pa产品。

• ***-ketoglutarate (α-KG) and pyruvate (PA) from a single fermentation broth or enzymatic conversion solution, wherein said method comprises: (A) fermenting a single strain of yeast to produce α-KG and PA in a single microbial fermentation broth;(B) pretreating the single microbial fermentation broth or an enzymatic conversion solution comprising both α-KG and PA by: centrifuging and separating microorganisms and other visible solids from the microbial fermentation broth or enzymatic conversion solution to obtain a supernatant,removing macromolecular impurities though a filtration process by filtering the supernatant through an ultrafiltration membrane module to remove macromolecular substances, cell fragments, pigments, lipids, proteins, and polysaccharides, and washing residual filtrate with a small amount of water, wherein the ultrafiltration membrane module is selected from the group consisting of: a roll membrane, a tubular membrane, and a plate membrane with molecular weight cut-off of 500 to 3,000 Daltons,concentrating α-KG and PA to a concentration of ≥120 g/L by decompression distillation at −*** to −*** Mpa and at a temperature of 50° ***° C., andseparating the concentrated α-KG and PA by basic ion exchange resin chromatography to remove high-valent cations thereby obtaining a pretreatment solution;(C) separating crude α-KG and crude PA by acidifying the pretreatment solution to obtain an acidified solution, wherein acidifying the pretreatment solution further comprises adjusting the pH of the pretreatment solution to be ≤*** by adding sulfuric acid while stirring;(D) extracting α-KG and PA to obtain an extraction solution from the acidified solution using a water-insoluble extractive agent by adding the water-insoluble organic solvent at 5° ***° ***, while maintaining a volume of extractant of ≥2-fold that of the acidified solution, and wherein extraction is repeated two more times;(E) determining a ratio of α-KG concentration to PA concentration in the extraction solution; wherein if Cα-KG:CPA is between 2 and *** in the extraction solution, then separating α-KG and PA by steps G-1 to K-1: G-1: further concentrating the extraction solution to obtain a higher concentration of α-KG by evaporation by concentrating α-KG to a concentration of ≥200 g/L by distilling a water-immiscible extractant, wherein the water-immiscible extractant is ethyl acetate or butyl acetate, under conditions of −*** to −*** Mpa and 35° ***° C., andH-1: cooling the concentrated liquid from step G-1 rapidly and crystallizing at low temperature of ≤15° ***, and separating the crude α-KG crystals and crude PA liquid by centrifuging after crystallization;I-1: washing crude crystals of α-KG obtained from the step H-1 with the water-immiscible organic solvent, wherein the water immiscible organic solvent is ethyl acetate or butyl acetate, with a volume of the water-immiscible organic solvent to be ≤½-fold of that of crude products; and harvesting the α-KG by leaching with the water-immiscible organic solvent, drying at a temperature of 50° ***° C., and crushing into powder;J-1: harvesting crude PA by distilling off extractant and water therein from liquid of PA gained from the step H-1, under conditions of −*** to −*** Mpa and at a temperature of 35° ***° C.;K-1: obtaining pure PA by distilling the pyruvate from the above steps by applying a high vacuum distillation or molecular distillation under conditions of −*** to −*** Mpa and at a temperature of 25° ***° C.;wherein if Cα-KG:CPA is less than 2 in the extraction solution, then: removing the extractive agent and the dissolved water therein by distillation,harvesting part of a pure PA with further distillation,adding a small amount of water to a residue of distillation, andseparating crude α-KG crystals and remaining crude PA liquid by centrifuging after crystallization at low temperature; and(F) purifying PA by distilling crude PA liquid under decompression conditions.
• ***, wherein pretreating the microbial fermentation broth or the enzymatic conversion solution further comprises one or more of: centrifugation, ultrafiltration, microfiltration, and filtration.
• ***, wherein the water-insoluble extractive agent is ethyl acetate or butyl acetate.
• ***, further comprising: determining a ratio of α-KG concentration to PA concentration in the extraction solution; wherein if Cα-KG:CPA is less than 2, then G-2: distilling off the extractant and water therein at −*** to −*** Mpa and 35 to 60° C.;H-2: obtaining a partial pure pyruvate by applying a high vacuum distillation or molecular distillation under conditions of −*** to −*** Mpa and 25 to 60° C.;I-2: separating crude α-KG crystals and crude pyruvate liquid after adding water to dissolve any residual solids completely and crystallizing at a temperature of 15° ***;J-2: obtaining the crude crystals of α-KG from the above step I-2 by washing with a water-immiscible organic solvent of ethyl acetate or butyl acetate, at a volume of ½-fold that of crude products; and harvesting pure α-KG after leaching with water-insoluble organic solvent, drying at 50 to 80° C., and crushing into powder;K-2: harvesting crude pyruvate by distilling off extractant and water therein from the crude pyruvate gained from the step I-2, at −*** to *** Mpa and 35 to 60° C.;L-2: obtaining pure pyruvate by distilling the crude pyruvate from the step K-2 with high vacuum distillation or molecular distillation at −*** to −*** Mpa and 25 to 60° C.
• ***, wherein the extraction is performed on the single microbial fermentation broth produced by the single strain of yeast, and wherein the single strain of yeast is a single strain of Yarrowia lipolytica or a single strain of Candida glabrata.
• ***, wherein the extraction is performed on the single microbial fermentation broth produced by the single strain of Yarrowia lipolytica, which is Yarrowia lipolytica WSH-Z06 (CCTCC M20714).
• ***, wherein the extraction is performed on the single microbial fermentation broth produced by the single strain of Candida glabrata, which is Candida glabrata CCTCC M202019.
• ***, wherein if Cα-KG:CPA is less than *** in the extraction solution, then the following steps are taken in step (E): removing the extractive agent and the dissolved water therein by distillation,harvesting part of a pure PA with further distillation,adding a small amount of water to a residue of distillation, andseparating crude α-KG crystals and remaining crude PA liquid by centrifuging after crystallization at low temperature.